Cleared-tissue direct-view oblique plane microscopy
Welcome! This website describes cleared-tissue direct-view oblique plane microscopy (CtDvOPM), developed at the MRC Laboratory of Molecular Biology, Cambridge, UK, and described in the manuscript index-agnostic oblique plane light sheet microscopy of centimetre-scale cleared tissues at subcellular resolution.
An extension of our original direct-view oblique plane microscopy approach, CtDvOPM is an index-agnostic approach for rapid, volumetric imaging of cleared tissues at subcellular resolution. In the current implementation, samples as large as 1680 mm3 can be imaged at 3.8 μm (XY) by 13.6 μm resolution (Z), with a simple modification providing 2 μm lateral resolution.
This website provides details of the hardware and software used, including an interactive CAD render and parts list. Example images are shown below at 1080p resolution — please click on them to view full-size images.
Example images
